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Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate

Scientific Journals of Bogor Agricultural University

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Title Extracellular Protease Activity of Enteropathogenic Escherechia coli on Mucin Substrate
 
Creator BUDIARTI, SRI
MUBARIK, NISA RACHMANIA
 
Description Enteropathogenic Escherichia coli (EPEC) causes gastrointestinal infections in human. EPEC invasion was initiated by attachment and aggressive colonization on intestinal surface. Attachment of EPEC alter the intestine mucosal cells. Despite this, the pathogenic mechanism of EPEC infectior has not been fully understood. This research hypothesizes that extracellular proteolytic enzymes is necessary for EPEC colonization. The enzyme is secreted into gastrointestinal milieu and presumably destroy mucus layer cover the gastrointestinal tract. The objective of this study was to assay EPEC extracellular protease enzyme by using mucin substrate. The activity of EPEC extracellular proteolytic enzyme on 1% mucin substrate was investigated. Non-pathogenic E. coli was used as a negative control. Positive and tentative controls were Yersinia enterocolitica and Salmonella. Ten EPEC strains were assayed, seven of them were able to degrade mucin, and the highest activity was produced by K1.1 strain. Both positive and tentative controls also showed the ability to digest 0.20% mucin. Key words: EPEC, protease, mucin, diarrhea
 
Publisher Bogor Agricultural University, Indonesia
 
Contributor
 
Date 2009-12-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/235
 
Source HAYATI Journal of Biosciences; Vol 14, No 1 (2007): March 2007; 36
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/235/101