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Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

Scientific Journals of Bogor Agricultural University

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Title Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity
 
Creator Hidayat, Topik; Indonesia University of Education
Pancoro, Adi; Bandung Institute of Technology
 
Description In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.
 
Publisher Bogor Agricultural University, Indonesia
 
Contributor
 
Date 2010-03-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/306
 
Source HAYATI Journal of Biosciences; Vol 17, No 1 (2010): March 2010; 50
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/306/167