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Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes

Scientific Journals of Bogor Agricultural University

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Title Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes
 
Creator WIDJIATI, .; Faculty of Veterinery Medicine, University of Airlangga
BOEDIONO, ARIEF; Faculty of Veterinery Medicine, Bogor Agricultural University
SUMITRO, SUTIMAN BAMBANG; Faculty of Mathematic and Natural Sciences, University of Brawijaya
HINTING, AUCKY; Faculty of Veterinery Medicine, University of Airlangga
AULANI’AM, .; Faculty of Mathematic and Natural Sciences, University of Brawijaya
SUSILOWATI, TRINIL; Faculty of Mathematic and Natural Sciences, University of Brawijaya
 
Subject transforming growth factor-β (TGF-β); cumulus oocyte complexes (COCs); identification
 
Description Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.
 
Publisher Bogor Agricultural University, Indonesia
 
Contributor
 
Date 2012-04-17
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/4818
 
Source HAYATI Journal of Biosciences; Vol 19, No 1 (2012): March 2012; 6
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/4818/3272