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Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase

Scientific Journals of Bogor Agricultural University

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Title Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase
 
Creator ARTIKA, I MADE; Bogor Agricultural University
 
Description The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to subunit 8 protein potentially facilitate elucidation of its topology, structure, and function. It has been shown that following incorporation of FLAG tag to its C-terminus, subunit 8 still assemble into functional ATP synthase complex. In order to analyze bioenergetic consequences of the FLAG tag addition, a yeast strain expressing FLAG tagged-subunit 8 was subjected to cellular respiration assays. Results obtained showed that addition of FLAG tag to the C-terminus of subunit 8 does not impair its proper functioning. The FLAG tag system, therefore, can be employed to study subunit 8’s detailed structure, topology, and function.
 
Publisher Bogor Agricultural University, Indonesia
 
Contributor Bogor Agricultural University, Indonesia
 
Date 2010-09-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/1693
 
Source HAYATI Journal of Biosciences; Vol 17, No 3 (2010): September 2010; 151
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/1693/736