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Genetic Diversity of Klebsiella spp. Isolated from Tempe based on Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR)

Scientific Journals of Bogor Agricultural University

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Title Genetic Diversity of Klebsiella spp. Isolated from Tempe based on Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR)
 
Creator BARUS, TATI; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
HANJAYA, IVAN; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
SADELI, JOANITA; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
LAY, BIBIANA W; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
SUWANTO, ANTONIUS; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia

Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia
YULANDI, ADI; Department of Biology, Faculty of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
 
Subject Tempe; Klebsiella; ERIC-PCR; vitamin B12
 
Description Tempe is an Indonesian fermented food prepared by fermenting dehulled cooked soybeans with Rhizopus oligosporus. Many types of bacteria are also involved during tempe fermentation, and one of these is Klebsiella spp.  Some isolates of K.  pneumoniae produces vitamin B12 in tempe but it has also been classified as an opportunistic pathogen. For this reason Klebsiella spp. in tempe is important to be studied. The aim of this study was to investigate the genetic diversity of Klebsiella spp. from tempe employing ERIC-PCR method. Sixty-one isolates of Klebsiella have been isolated from sixteen tempe producers  in Bogor, Jakarta, Malang, Tengerang, Bandung and Cianjur. 63F and 1387R primers were used to amplify 16S rDNA sequences, and 1R and 1F primers were used for ERIC analysis. The results of this research showed that sixty-one strains of Klebsiella were clustered into 17 groups. Based on ERIC-PCR analysis, isolates of Klebsiella could be grouped into different profiles which some of these groups consisted of isolates with identical ERIC-PCR profiles. Several identical ERIC-PCR profiles were found in tempe from the same producer. There was no correlation observed between genetic similarity  among isolates with the origin of tempe.
 
Publisher Bogor Agricultural University, Indonesia
 
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Date 2013-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/7320
 
Source HAYATI Journal of Biosciences; Vol 20, No 4 (2013): December 2013; 171-176
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/7320/pdf_1