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Yeast Isolation for Bioethanol Production

Scientific Journals of Bogor Agricultural University

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Title Yeast Isolation for Bioethanol Production
 
Creator RURIANI, EKA; Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
SUNARTI, TITI CANDRA; Department of Agroindustrial Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
MERYANDINI, ANJA; Biology Department, Faculty of Mathematic and Natural Science, Bogor Agricultural University
 
Subject yeast; glucose-xylose substrate: ethanol
 
Description We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.
 
Publisher Bogor Agricultural University, Indonesia
 
Contributor
 
Date 2012-10-15
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/hayati/article/view/5632
 
Source HAYATI Journal of Biosciences; Vol 19, No 3 (2012): September 2012; 145
2086-4094
1978-3019
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/hayati/article/view/5632/4271